John Stith Pemberton was born July 8, 1831, in Knoxville, Georgia. He spent most of his childhood in Rome, Georgia. His parents were James C. Pemberton and Martha L. Gant.
He entered the Reform Medical College of Georgia in Macon, and in 1850, at the age of nineteen, he was licensed to practice pharmacy however his main talent was chemistry.
Shortly thereafter, he met Ann Eliza Clifford Lewis of Columbus, Georgia, known to her friends as "Cliff", who had been a student at the Wesleyan College in Macon.
They were married in Columbus in 1853.
Their only child, Charles Ney Pemberton, was born in 1854.
They lived in the Pemberton House in Columbus.

He entered the Reform Medical College of Georgia in Macon, and in 1850, at the age of nineteen, he was licensed to practice pharmacy however his main talent was chemistry.
Shortly thereafter, he met Ann Eliza Clifford Lewis of Columbus, Georgia, known to her friends as "Cliff", who had been a student at the Wesleyan College in Macon.
They were married in Columbus in 1853.
Their only child, Charles Ney Pemberton, was born in 1854.
They lived in the Pemberton House in Columbus.